To enable accurate SNR quantification, consistent imaging settings that produced non-saturated pixels at all time points were selected. Fellowship support for K. All in vivo experiments were performed with five experimental replicates unless otherwise noted. In addition, we needed the new filters to block reflected light from LED illumination. Testing in model skin in strong light showed the 4-nanometer dots should be readable for at least five years. Toluene was then allowed to dry. In addition, photobleaching may also be enhanced in this experiment since seven-fold more intense light results in greater energy transfer per time, potentially enabling QD degradation to proceed more quickly.